HMGB1-mediated transcriptional activation of circadian gene TIMELESS contributes to endometrial cancer progression through Wnt-ß-catenin pathway.

TIMELESS (TIM) is a circadian gene which is implicated in the regulation of daily rhythm, DNA replication and repair, and cancer initiation and progression. Nevertheless, the role of TIM in endometrial cancer (EC) development is largely unknown. Bioinformatics analysis showed that TIM was aberrantly up-regulated in EC tissues and positively correlated with clinical or histological grade of EC. Functional studies showed that TIM knockdown reduced EC cell viability and restrained EC cell migration in vitro, as well as blocked xenograft tumor growth in vivo. Mechanistically, HMGB1 transcriptionally up-regulated TIM expression in EC cells. In addition, TIM could activate the transcription of the canonical Wnt ligand WNT8B, and TIM depletion could reduce the malignant potential of EC cells largely by targeting and down-regulating WNT8B. As a conclusion, HMGB1/TIM/WNT8B signal cascade was identified in this study for the first time. HMGB1 exerted its oncogenic role by activating the transcription of TIM, leading to the activation of Wnt signaling and EC progression.

Pilot-scale study of innovative mechanically-enhanced dynamic composting for treating kitchen waste.

This study introduced a novel mechanically-enhanced dynamic composting (MEDC) method for treating kitchen waste (KW) through partial-mixing and stratified fermentation. A pilot test varied aeration frequencies (AF) to refine control parameters and explore the maturation mechanism. Results showed that a moderate AF (10 min/4 h) achieved optimal efficiency, with a compost germination index of 123 % within 15 d. Moderate AF enhanced the growth of Corynebacterium_1 (25.4 %) and Saccharomonospora (10.5 %) during the low-temperature stage and Bacillus growth (91.3 %) during the maturation stage. Moreover, it enhanced microbial interactions (with an average degree of 19.9) and promoted substrate degradation and transformation, expediting heating and maturation. Multivariate dimensionality reduction analysis showed the MEDC accomplished rapid composting through stratified composting, dividing the reactor into distinct functional zones: feeding, low-temperature, high-temperature, and maturation. This enabled efficient microorganism enrichment and material degradation, expediting KW decomposition and maturation. This study offers a promising alternative for accelerated KW composting.

Regionalized life-cycle monetization can support the transition to sustainable rural food waste management in China.

Innovative recycling technologies can help curb food waste, yet their implementation often involves trade-offs among different environmental issues and among environmental, economic and social issues. Monetization can provide a solution to integrate all environmental impacts across the life cycle of food waste and to enable a normalized evaluation with economic accounting. Herein, a Chinese regionalized monetization model was applied to various indicators related to the environment, resource depletion and human health to assess ten typical rural food waste recycling technologies in Zhejiang province. The results reveal that biodrying and maturity and two bioconversion options are promising solutions, considering both environmental and economic impacts as well as the shifting of environmental impacts among different compartments as hidden risks. The monetization method proposed here could be applied to other sectors to support decision-making towards more sustainable development.

Pilot-scale study of step-feed anaerobic coupled four-stage micro-oxygen gradient aeration process for treating digested swine wastewater with low carbon/nitrogen ratios.

This study developed an innovative step-feed anaerobic coupled four-stage micro-oxygen gradient aeration process to treat digested swine wastewater. An anaerobic zone was used for prepositive denitrification; four micro-oxygen reactors (zones O1-O4) were used for simultaneous partial nitrification and denitrification through low-dissolved oxygen gradient control, step-feed, and swine wastewater-digested swine wastewater distribution. The nitrogen-removal efficiency was satisfactory (93 ± 3 %; effluent total nitrogen, 53 ± 19 mg/L). Mass balance coupled with quantitative polymerase chain reaction analysis revealed that simultaneous partial nitrification and denitrification was achieved in four micro-oxygen zones. Zones O1 were the major denitrification zones for nitrogen removal; nitrification was primary happened in zones O2 and O3. Correlation analysis confirmed that low-dissolved oxygen gradient control was the key to achieving efficient nitrogen removal. This study provides a low oxygen energy consumption method to treat digested swine wastewater with a low carbon/nitrogen ratio (<3).

Reducing nitrogen loss during kitchen waste composting using a bioaugmented mechanical process with low pH and enhanced ammonia assimilation.

Exploring the regulation of nitrogen transformation in bioaugmented mechanical composting (BMC) process for rural kitchen waste (KW) is essential to avoid the "not-in-my-backyard" phenomenon caused by nitrogen loss. Herein, nitrogen transformation and loss in BMC versus conventional pile composting (CPC) of KW were compared. The results showed that the total nitrogen loss in the BMC was 6.87-39.32 % lower than that in the CPC. The main pathways to prevent nitrogen loss in the BMC were reducing NH3 by avoiding a sharp increase in pH followed by transforming the preserved NH4+-N into recalcitrant nitrogen reservoir via enhanced ammonia assimilation. The enriched thermophilic bacteria with mineralization capacities (e.g., Bacillus and Corynebacterium) during rapid dehydration and heating in the BMC accumulated organic acids and easy-to-use carbon sources, which could lead to lower pH and ammonia assimilation enhancement, respectively. This study provides new ideas for formulating low-cost nitrogen conservation strategies in decentralized KW composting.

Carbohydrate degradation contributes to the main bioheat generation during kitchen waste biodrying process: A pilot study.

Biodrying is a promising method for processing kitchen waste (KW) with high moisture content into reusable solid recovered fuels (SRFs). During biodrying, a large amount of bioheat generated from biodegradation of biochemical components results in KW dehydration. However, the degradation rules of these components and their contribution to the bioheat in KW biodrying have not been systematically clarified. Here, a pilot experiment was performed to investigate the variations in biochemical components, hydrolase activities, and bioheat generation during three successive cycles of biodrying processes. Results showed that KW could be rapidly converted into SRFs with low calorific values of 6705-7062 kJ/kg and moisture content of 31.26%-35.21%. Analyses of hydrolase activities and mean fluorescence intensity suggested that the biodrying process pioneered the degradation of lipids and proteins in the warming stages, while carbohydrates (i.e. amylum, celluloses, etc.) underwent rapid decomposition in a large extent in the high-temperature and cooling stages. Carbohydrates with minimal difficulty in degradation, contributed 73.37%-89.92% to the total degradation mass and 59.23%-60.80% to the bioheat source during the three-cycle biodrying process. The generated bioheat was 4.32-4.56 times the amount of the theoretical heat used for water removal, indicating that internal bioheat could significantly enhance water evaporation and was sufficient for the expected water removal mass. Therefore, the evaluation of the main components to bioheat generation and its utilization efficiency makes a prominent contribution that can greatly clarify the conversion of KW biodrying into SRFs in order to efficiently promote renewable bioenergy and support the bioeconomy.

Dynamics of antibiotics and antibiotic resistance genes in four types of kitchen waste composting processes.

Kitchen waste might be a potential source of antibiotics and antibiotic resistance genes. Composting is recognized as an effective way for kitchen waste disposal. However, the effects of different kitchen waste composting types on the removal of antibiotics and antibiotic resistance genes haven't been systematically studied. In this study, the dynamics of antibiotics and antibiotic resistance genes from kitchen waste of four composting processes were compared. Results showed that although kitchen waste was composted, it remained an underestimated source of antibiotics (25.9-207.3 µg/kg dry weight) and antibiotic resistance genes (1012-1017 copies/kg dry weight). Dynamic composting processes (i.e., dynamic pile composting and mechanical composting) decreased the antibiotic removal efficiency and increased the abundance of some antibiotic resistance genes (5.35-8534.7% enrichment). Partial least-squares path model analysis showed that mobile genetic elements played a dominant role in driving antibiotic resistance genes dynamics. Furthermore, redundancy analysis revealed that temperature, pH, and water content considerably affected the removal of antibiotics and mobile genetic elements. This study provides further insights into exploring the effective strategies in minimizing the risk of antibiotic resistance from kitchen waste via composting process.

Feasibility of anaerobic digestion on the release of biogas and heavy metals from rice straw pretreated with sodium hydroxide.

The feasibility of anaerobic digestion on the release of biogas and heavy metals from contaminated rice straw pretreated with NaOH solution was studied. The results show that NaOH pretreatment can significantly boost the release of biogas and heavy metals from rice straw using anaerobic digestion. Under the optimal conditions for biomass pretreated 6% (w/w) NaOH with a solid-to-solution ratio of 1:20, total biogas and methane yields of 446.3 mL/g and 263.5 mL/g volatile solids were achieved, which were 22.18% and 41.59% higher than those of the control without NaOH pretreatment, respectively, and the release percentages of Cd, Pb, Cu, and Zn from rice straw reached 86.95-97.69%. The release of heavy metals from rice straw can contribute to both the degradation of lignin by NaOH pretreatment and the utilization/transformation of lignocellulose via anaerobic digestion. The acidification levels and total volatile fatty acid contents significantly influence on the release of heavy metals. Based on the Illumina HiSeq sequencing analysis, the dominant phyla in the biogas residues were proteolytic (Bacteroidetes) and hydrogen-producing (Firmicutes) bacteria, while the growth of Methanospirillum and Methanosaeta in anaerobically digested effluent was promoted. The results revealed that anaerobic digestion combined with NaOH pretreatment is suitable for the disposal of heavy metal-contaminated biomass.

Phytoextraction potential of Pteris vittata L. co-planted with woody species for As, Cd, Pb and Zn in contaminated soil.

The objective of this study was to determine the phytoextraction potential of a hyperaccumulator co-planted with a large biomass of woody plant in metal(loid)-contaminated soil. A pot experiment was conducted for 270 days (d) to study the growth, physiological responses, and metal(loid)s accumulation characteristics of plants, which included a shade-requiring, As-hyperaccumulator perennial herb, Pteris vittata L., co-planted with a woody tree, namely Morus alba L. or Broussonetia papyrifera L., for soil contaminated with arsenic (As), cadmium (Cd), lead (Pb), and zinc (Zn). The results showed that the biomass, photosynthetic pigment contents, antioxidant enzyme activity, and uptake of As in P. vittata L. were significantly enhanced by co-planting with M. alba L. or B. papyrifera L. Especially, the uptake of As by P. vittata L. was significantly (p < 0.05) increased by 80.0% and 64.2% when it was co-planted with M. alba L. or B. papyrifera L., respectively, while the As, Cd, Pb, and Zn contents of both M. alba L. and B. papyrifera L. were not significantly promoted by the co-planting. The comprehensive phytoextraction of metal(loid)s could be optimized by the co-planting of P. vittata L. with M. alba L. or B. papyrifera L. The total amount of As in the shoots from co-planting species was significantly (p < 0.05) higher than that of the monoculture with M. alba L. or B. papyrifera L., and that of Cd and Zn in the shoots was significantly (p < 0.05) higher than that of the monoculture with P. vittata L. The results showed that the co-planting of P. vittata L. with M. alba L. or B. papyrifera L. can alleviate the toxic effects of metal(loid)s on plant growth and improve the comprehensive phytoextraction amounts of metal(loid)s, and is a promising strategy for remediation of metal(loid)-contaminated soil.

Synergetic effect of Na-doping and carbon coating on the electrochemical performances of Li3-x Na x V2(PO4)3/C as cathode for lithium-ion batteries.

Carbon coated Li3-x Na x V2(PO4)3/C (x = 0.04, 0.06, 0.10, 0.12, 0.18) cathode materials for lithium-ion batteries were synthesized via a simple carbothermal reduction reaction route using methyl orange as the reducing agent, which also acted as the Na and carbon sources. The influence of various Na-doping levels on the structure and electrochemical performance of the Li3-x Na x V2(PO4)3/C composites was investigated. The valence state of vanadium, the form of residual carbon and the overall morphology of the Li2.90Na0.10V2(PO4)3/C, which showed the highest initial specific discharge capacity of 128 mA h g-1 at the current density of 0.1C (1C = 132 mA g-1) among this series of composites, were further examined by X-ray photoelectron spectroscopy, Raman spectroscopy, scanning electron microscopy and high-resolution transmission electron microscopy, respectively. The results indicated that a well crystallized structure of Na-doped Li2.90Na0.10V2(PO4)3 coated by a carbon matrix is obtained. In the further electrochemical measurements, the Li2.90Na0.10V2(PO4)3/C cathode material shows superior discharge capacities of 124, 118, 113, 106 and 98 mA h g-1 at 0.3, 0.5, 1, 2 and 5C, respectively. High capacity retention of 97% was obtained after 1100 cycles in long-term cyclic performance tests at 5C. The reason for such a promising electrochemical performance of the as-prepared Li2.90Na0.10V2(PO4)3/C has also been explored, which revealed that the synergetic effect of the Na-doping and carbon coating provide enlarged Li+ diffusion channels and the increased electronic conductivity.

Feasibility of anaerobic digestion for contaminated rice straw inoculated with waste activated sludge.

Anaerobic digestion of the heavy metal-contaminated rice straw inoculated with waste activated sludge was carried out through an orthogonal array. The results indicated that the digestion process was optimized with an inoculum to substrate ratio (I/S) of 0.25, a total solid (TS) content of 5%, and an initial pH of 7.0, with corresponding total biogas and methane yields of 745.4 and 285.2 mL/g VS, respectively. Under the optimized conditions, the bioleaching percentages of Cd and As in the biomass were 13.13% and 42.90%, respectively. According to range analysis, the biogas yield, methane yield, and bioleaching percentages of Cd and As in the biomass significantly decreased with an increase in TS content from 5% to 15%. The results suggested that anaerobic digestion with waste activated sludge was feasible for achieving both biogas production and bioleaching of heavy metals from contaminated rice straw.